6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. 8–250 nM) was measured as follows: the prothrombinase reaction proceeded for 3 min and was stopped by the addition of 10 mM EDTA. The GH Pro 340 Supplemental Greenhouse LED grow lighting is designed with greenhouse operations in mind. The antibodies originally defining this molecule by inducing apoptosis, anti-Fas and anti-APO-1 , were of the IgM or IgG3 subtype, respectively. SpectraMax 340 Absorbance Microplate Reader, Molecular Devices (CAT#: STEM-SPO-0021-ZJF) Labsystems Fluoroskan II Fluorescent Microplate Reader, Excitation Wavelength Range 320 – 700 nm, Labsystems (CAT#: STEM-SPO-0006-LGZ) BioTek MQX200 Microplate Reader Spectrophotometer, 24VDC, BioTek Instruments, Inc (CAT#: STEM. 2. application or budget. The absorbances were transformed to velocity of substrate consumption (nmol/min). Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 312 gain calibration failed. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)automated SpectraMAX 340 microtiter plate reader. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. The extinction coefficient at 280 nm used for determining the concentration of Rsp3690 was calculated to be 28,795 M –1 cm –1. 1 mL of acetylacetone. 05 ng/mL; progesterone: 2. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. SpectraMax Plus 384 measuring instruments pdf manual download. 5% (w/v) sodium dodecyl sulfate-polyacrylamide gels, and transferred to immobilon polyvinylidenedifluoride membranes (Millipore, Bedford, USA). mums. Samples were analyzed in triplicates. SpectraMax 340 (Obsolete), SpectraMax 340PC 384 (Obsolete), Gemini EM reader, SpectraMax 250 (Obsolete) What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full. Wavelength Range. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Microplate Reader Shelf. chen@case. Then the samples were incubated for 1 h with human IL6 detecting antibody and then with HRP conjugate for 40 min. FTIR Spectrometer: Shimadzu IRSpirit (2018)The data were acquired with a Molecular Devices SpectraMax 340 Microplate Reader (Sunnyvale, CA, USA). The cell concentration was measured by a spectrometer (SpectraMax 340, Molecular Devices, USA) with an optical density at 600 nm (OD 600). 4) was followed according to Vanacker et al. Enzyme activities at lower concentrations were assayed by using a LS50B fluorometric. Features demonstrated by the model include temperature control, a robot-compatible interface and data analysis software. CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). Quick View. 1 mM MnCl2 and 25 mM CHES buffer, pH 9. 0. control. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. PerkinElmer. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. TDP-43 stress granular cells were treated with 70% ice-cold. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. Data were expressed as the mean percent viable cell vs. Data are expressed as percent of maximum binding for each protein. ScanLater Western Blot Detection Cartridge. Wells were washed three times. Molecular Devices Spectramax 340PC instructions. It includes both a lamp and a new lamp holder. SpectraMax Injector Cartridge with SmartInject™ Technology. 5. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. addresses the needs of life science researchers who quantify very small amounts of DNA, RNA, oligos, and proteins. Data were expressed as the mean LPS-treated cells (100%) vs. Microplate Readers. Endpoint, kinetic, spectrum. Liquid handling for random screening was carried out with a Biomek FX from Beckman Coulter (Fullerton, CA, USA) and HYTRA. SpectraMAX 340 microtiter plate reader. This assay measures the two major sugars in wine, glucose and fructose. Softmax PRO v5. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. TheSpectraMax®Plus384addstheabilitytoread SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 0 2. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the visible range. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). These models can no longer be repaired. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). The SpectraMax® QuickDrop™ Micro-Volume Spectrophotometer . 85%. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. The protein concentration was determined spectrophotometrically at 280 nm in a 1 cm quartz cuvette using a SPECTRAmax-340 UV–vis spectrophotometer. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of. Plasma glucose levels were measured using FreeStyle Lite (Abbott Diabetes Care Ltd. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. All data were normalized to experimental day 0. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). Background: A computerized machine, SPECTRAmax 340, was used to evaluate the recently reported phenomenon of antagonism of the polyene amphotericin B (AMB) in Candida albicans pre-exposed to the triazole fluconazole (FLZ). 305 reference level saturation. This light offers spectrum variability, wireless control, and photosensors to automatically balance output with natural light. Cell permeabilization. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. 001/min at 425 nm. 2. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536 The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. This reader only allows high performance HTRF measurement when assays are run in. Molecular Devices VersaMax Absorbance Microplate Reader, 340-850 nm, Molecular Devices (CAT#: STEM-SPO-0005-YJL) Labsystems Fluoroskan II Fluorescent Microplate Reader, 320 – 700 nm. \r plates can be used in the SPECTRAmax PLUS 384. using SpectraMax i3 (Molecular devices, San Jose, USA). Briefly, 50 µl-samples were incubated with equal volumes of Griess reagent (1% sulphanilamide, 0. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. . The Catalina. a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). The values were compared against a standard curve that was generated using known concentrations of IL6 to calculate concentration in the. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). Spectramax 340pc User Manual, Hudson River Saranac Pellet Stove Manual, Alesis Midiverb 1 Manual, User Manual For Samsung Galaxy Ace Duos S6802, Di Style Manual, R150f Transmission Repair Manual, Eos5d Mark 2 Manual. Revvity. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. Or scan up to 384 wells at any time to determine the best assay conditions. using SpectraMax i3 (Molecular devices, San Jose, USA). Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 1% N-1-naphtylethylenediamine dihydrochloride, 2. Immunofluorescence and Aggregation Analysis. more on but is otherwise untested and sold AS-IS. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). AoSMCs were seeded into 6-well culture plates. SoftMax Pro. The reaction was then stopped by adding 50 µL of 1 M sulfuric acid to each well. Temperature Range: Ambient + 4 °C up to 45 °C. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. Acceptor filter: 665/10 nm. All absorbance measurements were taken on a SpectraMax 340 PC reader equipped with SoFTMax Pro software. SpectraMAX 340. Growth was monitored at 30°C every 6 min for 8 to 12 h at 660 nm using a multiscan photometer (spectraMax 340; Molecular Devices). Money Saved: $340. 1 mL of acetylacetone. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. • TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027: EX range: 340/80 nm EM range: 616/10 nm: High fg levels of Streptavidin: High. 4) was followed according to Vanacker et al. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. The manufacturer of the SpectraMax 340PC Microplate Spectrophotometer is Molecular Devices. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. Molecular Devices SpectraMax Gemini XS Microplate Reader. Samples containing 25 μg of protein were separated on 12. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter $1,000. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. 5 mL of 1 M acetate buffer (pH 4. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Molecular Devices SPECTRAmax. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. (1993). using SpectraMax i3 (Molecular devices, San Jose, USA). The SpectraMax 384 Plus lets you get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. Details. Stat Fax 3200 Microplate Reader. SpectraMax 340 from Molecular Devices). RESEARCH ARTICLE Effects of early-life conditions on innate immune function in adult zebra finches Merijn M. Awareness Technology Inc. Novak, Nathan J. 0 1. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). Please refer to photos for further. ELISAs were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). 5 - 2. The possi-ble effect of zinc or copper on the binding of heme by A was tested as described above by using a 50 and 100 M final concentration of each metal. SpectraMax Multi-Mode Microplate Readers. #N207) LAL Reagent Water (Lonza cat. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays. ac. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. 0. The metals were preincubated with A for 5 min when added before heme. No power cord or accessories included. FXII or PK activation by FXIa was tested as follows: FXII or PK (200nM) was incubated with or without FXIa (25nM) in TBS, 0. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance plate reader (Molecular Devices, Union City, CA). Irene Tieleman, Ido PenandSimon VerhulstSubsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 00 USD. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. After washing, the dye was eluted with 3% HCl, and the absorbance measured with a SpectraMax 340 microtiter plates reader at 665 nm. ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. Streamline GLP/GMP compliance. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. 5% phosphoric acid) in microplates at room temperature for 10 min. 316 grating motor driver faulty. Although all samples initially were analyzed at the same volume, the volume of sample. Statistical AnalysisConsult Molecular Devices's entire SpectraMax i3x catalogue on MedicalExpo. ir Neuroprotection of a sesamin derivative, 1, 2-bis [(3-methoxy- phenyl) methyl] ethane-1, 2-dicaroxylic acid (MMEDA) againstEach SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Microplate Readers. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. Service parts for these models are not being produced and are no longer in stock. The fluorescence intensity was measured using the SpectraMax 340 microplate reader (Molecular Devices) at the following conditions: excitation at 475 nm and emission at 529 nm (green fluorescence) or 590 nm (red fluorescence). 10. IMAP ® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. 1 mL of culture media with 10% FBS. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. SpectraMax Multi-Mode Microplate Readers. High fg levels of Streptavidin High fg levels of Streptavidin Exceeds Cisbio certification requirements TRF-based with 340/80 nm EX and 616/10 nm EM AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm. 1% Chaps, 10 m M dithiothreitol, and 10% sucrose. SpectraMax Multi-Mode Microplate Readers. 315 can't find zero order. SPECTRAMAX 340. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Like CD3, the CD95 death receptor requires binding of immobilized or multimeric antibodies to be activated effectively. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance platereader (Molecular Devices, Union City, CA, United States). TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross awiderangeofconcentrations. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. Product Keyword. or microplate reader (e. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Values are expressed as the mean s ± SD of 3 separate experiments, each performed in triplicate. 5 hours, and is measured in a 96-well plate spectrophotometer at 405 nm (SpectraMax 340; Molecular Devices LLC, Sunnyvale, CA, USA). The extreme flexibility and high sensitivity of the SpectraMax GEMINI EM make it appropriate for applications within the fields of bio-A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Donor: 616/10 nm. #N207) LAL Reagent Water (Lonza cat. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. SpectraMax QuickDrop Micro-Volume Spectrophotometer. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. 2 g/L solution; dilute 1:10 in HBSS. Or scan up to 384 wells at any time to determine the best assay conditions. Near bottom. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the. 8. Other technologies such as imaging. ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. The SPECTRAmax 340PC can measure. SpectraMax 190 reads 96-well and strip-well plates. , Sunnyvale, CA). used. Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). This is equivalent to having 511 built-in filters,Colour change was measured in a SpectraMAX ® 340 spectrophotometer. Western blot. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full bandwidth at 1/2 the transmission peak for the selected wavelength. SpectraMax Gemini EM & FLEXstation (bottom read) 1. ELISA plates were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). Values were determined according to a standard curve (log/log curve-fit). N-34-LB. Operators can conveniently select up to 6 wavelengths at a time for. Customer Transformation Hub. 2 M DEEMM were mixed in a microtube,. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 1. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. 6. The SpectraMax® iD3 Multi Mode Microplate . using SpectraMax i3 (Molecular devices, San Jose, USA). The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. Labsystems Luminoskan RT Microplate Reader. Examples: “Press carefully to engage,” “Do not press down. 024). Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 190–850 nm <± 1. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. SpectraMax 384 Plus. 5. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. 1. The area under the curve (AUC) for the comparison of the growth rates was determined using. Summary of Contents for Molecular Devices SPECTRAmax 340PC. Microplate Spectrophotometer Operator’s Manual. Number of flashes. Rate of fibrinolysis. Binding of capture antibodies to polystyrene beads and biotinylation. The cells were pretreated, serum starved for 24 h, and then treated with NK-cdM priora SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). 9000-0756. 6. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. Details. 340/70 nm. Description. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise results. 340 nm / 70 nm. Amino Acid Analysis. Molecular Devices Corporation 1311. Changes in cell viability were reflected by changes in optical density detected at 450 nm, which was measured using a spectrophotometer microplate reader (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027 EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm EX laser diode and a 570 nm (100) EM filter Pick best speed, sensitivity, and price for your needs Table 1. Upgradeable modules are also available including western blot, cell imaging, and fast. Each SpectraMax® 340 PC 384 Microplate Reader has an. Title: SPECTRAmax 340 Microplate Reader manual Author: Research Facilities Keywords: spectramax, 340, microplate reader, manual, Created Date: 12/13/2005 9:07:53 AMSpectraMax Multi-Mode Microplate Readers. Percent Transmission Absorbance Competitor 0. ATI/240, 250. Glutathione reductase (GR) activity was measured, according to Smith et al. These lab workhorses are used in drug discovery, research, bioassay validation, and. 003 OD Stray light ≤ 0. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). This is equivalent to having 511 built-in filters,Results: A total of 64% of the subjects reported improved quality of sleep during treatment with naloxone at a dose of 40 ng per 24 hours as compared with 9% with sham treatment (P=0. 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. Near bottom. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. MCE has not independently confirmed the accuracy of these methods. chen@case. Software (SoftMax Pro ® software or comparable plate reader software). CD154 is an established indicator of antigen-reactive T helper cells in other species, but has not been characterized in horses. Awareness Technology Inc. 00. 5. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The SpectraMax GEMINI EM cannot be operated without the computer and SoftMax Pro software. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Statistical analysis. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). Labsystems Luminoskan RT Microplate Reader. Analysis of ALA by Ehrlich’s Reagent Assay. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). Methods: We investigated growth inhibition by varying concentrations of AMB in seven isolates of C. The OD for each serum is adjusted by subtracting either the OD for spontaneous mediator release or antigen-specific mediator release. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. Briefly, ear or dorsal skin was dissected and fixed with 10% phosphate buffered formalin for 24–72 h. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). The ratio of red to green fluorescence intensity was calculated. 7. Each experiment was performed in duplicate and repeated at. Absorbance values were read at 570 nm on an automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA). The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. 6. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Italic and boldface type are used for emphasis. Male 8 pin mini Din to Female 9D. View the Molecular Devices website. Fibrin formation was measured by turbidity at 405 nm using a SpectraMax 340 PC plate reader. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Versteegh, Yoran H. SpectraMax 190 reads 96-well and strip-well plates. Immunofluorescence and Aggregation Analysis. 5 mm (bottom read) Beam diverges to focal spot near bottom of well. The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. Developed plates were read on MSD reader Sector Imager S 600, Model 1200, with Discovery Workbench Software (version 4. Operators can conveniently select up to 6 wavelengths at a time for absorbance detection in the visible wavelength range 340 nm - 850 nm. Alves, Amelia K. Supplier: Molecular Devices. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 340-850 nm: Wavelength Selection: Monochromator, turnable in 1 nm increments:. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. Fibrin clots were formed as described above with some modifications 46. Created Date: 20051213090753Zmeasured using a SpectraMax 340 multiwell plate. Wound healing assay. 304 signal level saturation. density was measured at 570 nm (SpectraMax 340, Molecular De-vices, Sunnyvale, CA). Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. , Sunnyvale, CA). The mixture changed to a red color by a reaction of LDH that was measured by a plate reader (SpectraMax 340 PC; Molecular Devices, San Jose, CA, USA) at a visible wavelength of. reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). Studies of the regulation of AoSMC migration by Angpt2 were preformed using a wound healing assay. Example: [Drawer]. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). StakMax Microplate Handling Stacker. . #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices) Methods VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. The narrow bandwidth used by the model increases measurement accuracy and broad analysis over. Absorbance was read at 450 nm using a Molecular Device Spectramax 340. Immunofluorescence and Aggregation Analysis. 5 g/L-EDTA 0. Cell Assay Cells (5 × 10 5 per well) are plated in triplicate in 96-well plates in 0. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. (1988) and recorded at 412 nm. Manager: Yinghua Chen, PhD Contact Email: yinghua. Samples with OD values of less than 0. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. Acceptor: 665/10 nm. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. TheSpectraMax®Plus384addstheabilitytoreadcuvettes. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). measurements were performed on a SPECTRAmax-340 plate reader (Molecular Devices Inc. If depot service is the best option for you we will assist you with logistics to help you keep. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Water – distilled water or equivalent. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the. , Sunnyvale, CA). 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. TGFα release was calculated as described in the Results. Wavelength Selection. Protein electrophoresis system (BioRad, Hercules, CA) and Odyssey Infrared Imaging System (Li-Cor Biosciences, Lincoln, NE) were used in western blot. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). 306 plate air cal. 6) and 0. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader.